Fractionation of Estrogen-Methyl-Esters with Alumina Column Chromatography

Paper chromatography of methyl esters of porphyrins.

A micromethod for detecting and determining various porphyrins extracted from biological materials, especially nervous tissues (I), is needed. The technique of filter paper chromatography by Consden, Gordon, and Martin (a), with modification by Williams and Kirby’s capillary ascent (3), has been applied to the problem in this study. The recent paper by Nicholas and Rimington (4) on partition ch...

The fractionation of cholesterol esters by silicic acid chromatography.

Within recent years, the technique of silicic acid chromatography to separate complex mixtures of lipides has been developed widely (1-3). At the present time it is possible to resolve some eight different classes of lipides ranging from hydrocarbons to phospholipides on a single column (4). In addition, this procedure has been used to separate classes of phospholipides from one another (5). Ou...

Fractionation of Cholesterol Esters by Thin-layer Chromatography.

Large-scale separation of fatty acid methyl esters by column chromatography on acid-washed Florisil impregnated with silver nitrate.

Preparation of fatty acid methyl esters for gas-liquid chromatography.

A convenient method using commercial aqueous concentrated HCl (conc. HCl; 35%, w/w) as an acid catalyst was developed for preparation of fatty acid methyl esters (FAMEs) from sterol esters, triacylglycerols, phospholipids, and FFAs for gas-liquid chromatography (GC). An 8% (w/v) solution of HCl in methanol/water (85:15, v/v) was prepared by diluting 9.7 ml of conc. HCl with 41.5 ml of methanol....